# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 14 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 14.0 246.1 24.1 0.3 19.2 0.0 14.1 142.4 35.4 0.3 30.3 0.3 14.2 87.8 45.7 5.2 40.9 5.3 14.3 49.4 61.1 0.1 58.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 15 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 15.0 197.7 24.0 0.1 18.8 0.1 15.1 141.3 33.9 0.1 28.4 0.0 15.2 101.1 43.5 0.3 39.4 0.2 15.3 66.0 52.2 0.2 47.3 0.2 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 16 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 16.0 994.3 26.0 0.1 20.6 0.0 16.1 816.9 29.4 0.1 23.9 0.1 16.2 779.6 32.3 0.1 26.7 0.1 16.3 685.5 36.4 0.0 30.7 0.0 16.4 600.1 39.4 0.2 33.8 0.0 16.5 531.1 41.9 0.0 37.0 0.3 16.6 445.7 47.2 0.0 41.6 0.0 16.7 427.1 48.8 0.1 44.6 0.3 16.8 303.3 59.1 0.3 53.0 0.1 16.9 217.9 68.9 0.1 61.6 0.2 16.1 129.2 80.3 0.2 72.8 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 17 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 17.0 878.2 26.0 0.0 20.7 0.1 17.1 779.6 31.2 2.2 23.8 0.4 17.2 676.7 34.0 0.0 28.3 0.1 17.3 574.9 38.3 0.2 32.4 0.1 17.4 463.2 44.5 0.0 38.2 0.1 17.5 357.0 52.1 0.0 45.4 0.2 17.6 298.9 59.5 0.0 52.7 0.1 17.7 224.5 65.2 0.0 58.3 0.0 17.8 162.1 72.8 0.0 65.4 0.2 17.9 117.2 78.2 0.0 71.5 0.2 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 18 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 18.0 834.4 24.9 0.2 19.6 0.1 18.1 789.5 26.8 0.0 21.4 0.1 18.2 757.7 28.6 0.1 23.2 0.1 18.3 720.5 30.9 0.0 25.5 0.2 18.4 665.8 33.4 0.1 27.9 0.0 18.5 563.9 37.2 0.0 31.5 0.0 18.6 487.3 41.0 0.0 35.3 0.0 18.7 431.4 44.5 0.1 38.4 0.0 18.8 422.7 47.2 0.2 41.0 0.0 18.9 330.7 53.4 0.0 47.0 0.1 18.1 283.6 58.7 0.0 52.2 0.1 18.11 214.6 65.1 0.1 58.1 0.3 18.12 182.9 70.4 0.0 63.4 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 19 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 19.0 985.5 25.2 0.0 19.9 0.0 19.1 842.1 28.1 0.1 22.8 0.2 19.2 765.4 31.6 0.1 25.9 0.2 19.3 671.2 35.0 0.0 29.5 0.1 19.4 570.5 39.2 0.1 33.3 0.2 19.5 514.7 42.5 0.0 36.4 0.2 19.6 441.3 45.8 0.1 39.6 0.2 19.7 420.5 48.6 0.0 42.4 0.1 19.8 319.7 56.7 0.2 49.6 0.2 19.9 283.6 62.5 0.1 55.6 0.2 19.1 233.2 64.0 0.1 59.5 0.0 19.11 210.2 67.6 0.1 61.1 0.3